Published October 2000
by John Wiley & Sons .
Written in English
|The Physical Object|
Chapters cover a wide-range of topics such as dealing with the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia co-culture models, and cell-based assays for the evaluation of neuroprotective molecules. Cell culture systems for specific neural cell types are thus essential for studies of their development and function. This laboratory manual provides step-by-step protocols for isolating specific cell populations from rodent tissues and culturing . A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation Noah Goshi1, Rhianna K. Morgan2, Pamela J. Lein2 and Erkin Seker3* Abstract Background: Interactions between neurons, astrocytes, and microglia critically influence neuroinflammatory responses to insult in the central nervous system. Aspects of developmental biology, the immune system, the nervous system, and plant biology are thus discussed in their broader biological context in chapters covering areas such as genome structure, gene expression, DNA rearrangements, the plasma membrane, cell signaling.
This book offers a wide-ranging look at the latest studies, including: foodborne pathogens, including how, when, and where bacteria interact with the gut and its microbiota; infections of the urogenital tract, endothelial barriers, and the nervous system. 12 Derivation and Culture of TS Cell Lines 13 Derivation and Culture of XEN Cell Lines 14 Derivation and Culture of EpiSC Lines 15 Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System 16 Isolation, Propagation, and Differentiation of Radial Glia-Like Neural Progenitor Cells . research which promisingly employs cell culture models is the study of in vitro drug transport/absorption and metabolism. Drug Delivery System Workshop August , . As well as delivering the RNA into neuronal cells in cell culture, an antiviral siRNA was delivered specifically into the brains of mice infected with encephalitis: about 80% of the mice Cited by:
In 3D models, cells can be cultivated in three dimensions like in their natural environment in tissues and organs. In neurosciences, 3D models are mostly devoted to the central nervous system to. The high porosity allowed for full 3D infiltration of neural cells. Using neural progenitor cells, they observed a highly integrated network, synaptogenesis, and extensive neurite outgrowth. Compared to the 2D culture, where neuronal cells grew on top of glial cells in separate layers, cells intermixed in 3D culture Cited by: 7. This book provides an expert view of major concepts in autophagy research with a focus on autophagy in neurons. Experimental evidence for evolutionarily conserved and specialized regulatory mechanisms for autophagy in the mammalian nervous system . The European Collection of Authenticated Cell Cultures (ECACC) The European Collection of Authenticated Cell Cultures (ECACC) was established in as a cell culture collection to service .